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Chemical methods to improve the dyeing properties of flax

State University Testing Center) Abstract: Using double diffusion principle, preparation pure silk fibroin gel, and its verification. The results show that the gel is a pure silk fibroin porous yellow sponge-like solid structure,[link widoczny dla zalogowanych], the structure contains a random coil, SF I, SF Ⅱ three different conformations, and at low temperature (a 4 ~ C) crystallization, the formation of silk fibroin gel more I conformation. Gel breaking strength depends on the moisture content. Keywords: winding ropes Preparation of gel structure and properties of CLC: TS102.33 Wenhui code: A frozen silk fibroin gel membrane is a porous membrane structure of biological proteins, which can be used as the growth of cells and tissues scaffold material, play support and promote the growth of cells and tissues, regulation and induction of cell and tissue differentiation and other important role in cell culture, blood vessels and other organs or tissue regeneration,[link widoczny dla zalogowanych], and intelligent drug delivery materials, food and chemical industry has potential applications ¨ l0 three-dimensional structure of the silk fibroin gel membrane is a new tissue engineering materials to be developed, mainly prepared by emulsion freeze-drying of porous silk fibroin gel, which first increases fibroin solution were appropriate crosslinking agent, then the solution freeze-drying operation of multiple gel membrane obtained. N1 Japanese scholars were prepared using the above fibroin gel crosslinked to study the mechanical properties of dry gel and the pore structure and freezing temperature, the relationship between the concentration of silk fibroin. We use the above method also successfully prepared fibroin gel crosslinked blink lo freeze-drying method has the advantage not easily denatured protein residue and impurities, the disadvantage is the expensive equipment, general laboratory unbearable,[link widoczny dla zalogowanych], we in the development process of the porous membrane , try to use ordinary refrigerator freezer fibroin solution as equipment, combined with wet film forming process of the double-diffusion theory, which uses a certain temperature,[link widoczny dla zalogowanych], the polarity of the solvent extraction frozen silk fibroin solution (solid), repeated several times, then the silk fibroin solution set and the refrigerator freezer is certain to ask, to continue to cycle the operation 3 times to 4 times,[link widoczny dla zalogowanych], and finally, he will be frozen porous silk fibroin gel. Not only provides a porous silk fibroin membrane prepared a new way, and more conducive to in-depth grasp of the performance of silk fibroin gel for the early realization of its widespread adoption. 1 Experimental 1.1 Preparation of silk fibroin solution by lanthanum were prepared by literature 【5】 fibroin solution containing 5.10% solid content. 1.2 Preparation of porous silk fibroin gel will be filled with a certain amount of silk fibroin solution in Petri dishes placed in the refrigerator freezer 72lI (a 4 ~ C), and then removed at room temperature, 30 ℃ with 30mL of acetone solvent pumping Tisi elements in the water, each 5rain a 8rain, a total of 3 to 4 times, finished, to continue to release the silk fibroin solution frozen at 72h. Cycle the operation 3 times to 4 times, then the system is much more porous silk fibroin gel. Loss rate of 1.3 dissolved the quality of the test will be known to thousands of gel were immersed in temperature l5 ℃ of 0.10mol / L hydrochloric acid (pH to 1.), 0.05mol / L potassium hydrogen phthalate (pH 4.01), distilled water (pH 6.75) and 0.025nml / L borax solution (pH 9.1 Cool

and, when asked after the required removal, said after drying to constant weight. 1.4 Sample preparation 1.4.1 Mechanical performance tests take a number of pieces of dry gel (6.0 × 2.0 × 0.18em) placed l5 ℃ distilled water for a while asked, siphoning off the sample surface with filter paper water droplets, will be dry and place it in a different time infrared lamp, weighed after cooling to room temperature and calculate the moisture content of the sample. 1.4.2 Test 'in INSI'RON Rally 1120 on a test breaking strength. Folder from the 4era, paper speed is 8 ~ mn / mill, the temperature 3nuno3. Staining method using reactive dyes used to characterize the modified and designed the L9 (3.) Orthogonal experiment, modification of process parameters optimization results show that the Modified exhaust optimum process conditions were: concentration of modifier for the 4 / L, NaOH concentration of 15g / L, bath ratio 1:20, temperature 60 ~ C, when asked to 60rain. '
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